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ProductsBack/IL2 Receptor beta antibody [MAB0831]

IL2 Receptor beta antibody [MAB0831]

GTX52419
GeneTex
ApplicationsFlow Cytometry, Neutralisation/Blocking
DownloadDatasheet
Product group Antibodies
TargetIl2rb
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL2 Receptor beta antibody [MAB0831]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MAB0831
  • Conjugate
    Unconjugated
  • Gene ID16185
  • Target name
    Il2rb
  • Target description
    interleukin 2 receptor, beta chain
  • Target synonyms
    CD122; high affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL15R; IL-15R; IL15Rbeta; IL-15Rbeta; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; Il-2/15Rbeta; Il-2R; IL-2R subunit beta; IL-2RB; Il-2Rbeta; interleukin-2 receptor subunit beta; p70; p70-75
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP16297
  • Protein Name
    Interleukin-2 receptor subunit beta
  • Scientific Description
    The interleukin 2 receptor is composed of alpha and beta subunits. The beta subunit encoded by this gene is very homologous to the human beta subunit and also shows structural similarity to other cytokine receptors. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL2RB using anti-IL2RB antibody (PB9761). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Spleen Tissue Lysate, Lane 3: Mouse Testis Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RB antigen affinity purified polyclonal antibody (Catalog # PB9761) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RB at approximately 75 kDa. The expected band size for IL2RB is at 75 kDa.
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