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ProductsBack/GenieFluor 488 Anti-Mouse CD122 Antibody [5H4]

GenieFluor 488 Anti-Mouse CD122 Antibody [5H4]

AGEL0146
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetIl2rb
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Overview

  • Supplier
    Assay Genie
  • Product Name
    GenieFluor 488 Anti-Mouse CD122 Antibody [5H4]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H4
  • Conjugate
    Other Conjugate
  • Gene ID16185
  • Target name
    Il2rb
  • Target description
    interleukin 2 receptor, beta chain
  • Target synonyms
    CD122, IL-15Rbeta, IL15Rbeta, Il-2/15Rbeta, Il-2Rbeta, p70, interleukin-2 receptor subunit beta, IL-15 receptor beta chain, IL-2 receptor beta chain, IL-2 receptor subunit beta, IL-2/15 receptor-beta, IL-2R subunit beta, IL-2RB, high affinity IL-2 receptor subunit beta, p70-75
  • Host
    Rat
  • Protein IDP16297
  • Protein Name
    Interleukin-2 receptor subunit beta
  • Scientific Description
    GenieFluor 488 Anti-Mouse CD122 Antibody [5H4] is a high-performance monoclonal that specifically targets Mouse CD122, delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Available with AF488 conjugation for enhanced detection sensitivity and experimental flexibility. Conveniently packaged in 100 Tests format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL2RB using anti-IL2RB antibody (PB9761). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Spleen Tissue Lysate, Lane 3: Mouse Testis Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RB antigen affinity purified polyclonal antibody (Catalog # PB9761) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RB at approximately 75 kDa. The expected band size for IL2RB is at 75 kDa.
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