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Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.

Anti-IL2RB Antibody

HPA062657
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetIL2RB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL2RB
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3560
  • Target name
    IL2RB
  • Target description
    interleukin 2 receptor subunit beta
  • Target synonyms
    CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL15RB; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IMD63; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-15 receptor subunit beta; interleukin-2 receptor subunit beta; P70-75; p75
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14784
  • Protein Name
    Interleukin-2 receptor subunit beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL2 Receptor beta/p75/IL2RB using anti-IL2 Receptor beta/p75/IL2RB antibody (A03401-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse intestine tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 Receptor beta/p75/IL2RB antigen affinity purified polyclonal antibody (Catalog # A03401-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 Receptor beta/p75/IL2RB at approximately 75KD. The expected band size for IL2 Receptor beta/p75/IL2RB is at 75KD.
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