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Phospho-IL2RB (Y364) Antibody

CSB-PA009546
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetIL2RB
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-IL2RB (Y364) Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3560
  • Target name
    IL2RB
  • Target description
    interleukin 2 receptor subunit beta
  • Target synonyms
    CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL15RB; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IMD63; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-15 receptor subunit beta; interleukin-2 receptor subunit beta; P70-75; p75
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14784
  • Protein Name
    Interleukin-2 receptor subunit beta
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL2 Receptor beta/p75/IL2RB using anti-IL2 Receptor beta/p75/IL2RB antibody (A03401-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse intestine tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 Receptor beta/p75/IL2RB antigen affinity purified polyclonal antibody (Catalog # A03401-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 Receptor beta/p75/IL2RB at approximately 75KD. The expected band size for IL2 Receptor beta/p75/IL2RB is at 75KD.
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