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Anti-ILK Antibody

Anti-ILK Antibody

A96448
Antibodies.com
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ILK Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to ILK.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (A02932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whhole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U20S whhole cell lysates, Lane 6: human PC-3 whhole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat PC-12 whhole cell lysates, Lane 11: mouse heart tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse skeletal muscle tissue lysates, Lane 14: mouse C2C12 whhole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin linked ILK antigen affinity purified polyclonal antibody (Catalog # A02932-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 51 kDa. The expected band size for Integrin linked ILK is at 51 kDa.
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Immunofluorescence staining of MCF-7 cells with CSB-PA00999A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & cell junctions.
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Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
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