Anti-integrin alpha5beta1 [7H5]

Absolute Antibody

Anti-integrin alpha5beta1 [7H5]

9

This antibody is specific for human integrin alpha5beta1.

The murine version of this antibody was used in a HUVEC direct binding assay. In this assay the ability of the antibody to bind to HUVACs was tested (US2012195908). Furthermore, the murine version of this antibody was also utilized in flow ctyometry to stain RAJI cells and HUVEC cells. It only bound HUVECs and not RAJI cells (US2012195908). Additionally, it was determined that the murine version of this antibody could inhibit cell adhesion to fibronectin (US2012195908). An HUVEC Caspase-3/7 Activity Colometric Assay was preformed using the murine version of this antibod to determine the apoptosis resulting from the administration of the antibody (US2012195908). HUVEC tube formation assays were preformed using the murine version of this antibody (US2012195908). The humanized version of this antibody was shown to bind to human recombinant integrin alpha5beta1 in ELISA (US2012148572). Furthermore, the binding affinity of the humanized version of this antibody was confirmed using surface plasmon resonance (US2012148572). Finally, a skin wound healing assay was preformed. The humanized version of this antibody showed inhibition of wound healing in white new zealand rabbits (US2012148572).

Functional Assay, Flow Cytometry, ELISA

Research Use Only

Monoclonal

7H5

ITGA5

integrin subunit alpha 5

CD49 antigen-like family member E; CD49e; fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; integrin alpha-5; integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5/SLC9B1 fusion; very late activation protein 5, alpha subunit; VLA-5; VLA5A

Mouse

IgG2a

Integrin alpha-5

Human

-20°C,2°C to 8°C

12352203