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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Anti-INTU Antibody

HPA036714
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetINTU
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-INTU
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27152
  • Target name
    INTU
  • Target description
    inturned planar cell polarity protein
  • Target synonyms
    ciliogenesis and planar polarity effector 4; CPLANE4; INT; inturned planar cell polarity effector homolog; OFD17; PDZ domain containing 6; PDZ domain-containing protein 6; PDZD6; PDZK6; protein inturned; SRTD20
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULD6
  • Protein Name
    Protein inturned
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INTU using anti-INTU antibody (A11533-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTU antigen affinity purified polyclonal antibody (Catalog # A11533-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTU at approximately 100 kDa. The expected band size for INTU is at 80,106 kDa.
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