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INTU Antibody

PACO60436
Assay Genie
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
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Product group Antibodies
TargetINTU
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Overview

  • Supplier
    Assay Genie
  • Product Name
    INTU Antibody (PACO60436)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID27152
  • Target name
    INTU
  • Target description
    inturned planar cell polarity protein
  • Target synonyms
    ciliogenesis and planar polarity effector 4; CPLANE4; INT; inturned planar cell polarity effector homolog; OFD17; PDZ domain containing 6; PDZ domain-containing protein 6; PDZD6; PDZK6; protein inturned; SRTD20
  • Host
    Rabbit
  • Protein IDQ9ULD6
  • Protein Name
    Protein inturned
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INTU using anti-INTU antibody (A11533-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTU antigen affinity purified polyclonal antibody (Catalog # A11533-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTU at approximately 100 kDa. The expected band size for INTU is at 80,106 kDa.
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