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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

Anti-JAG2 Antibody

HPA050567
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetJAG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-JAG2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3714
  • Target name
    JAG2
  • Target description
    jagged canonical Notch ligand 2
  • Target synonyms
    HJ2; jagged 2; protein jagged-2; SER2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y219
  • Protein Name
    Protein jagged-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Jagged 2/JAG2 using anti-Jagged 2/JAG2 antibody (A01428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Jagged 2/JAG2 antigen affinity purified polyclonal antibody (Catalog # A01428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Jagged 2/JAG2 at approximately 130 kDa. The expected band size for Jagged 2/JAG2 is at 133 kDa.
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