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Immunohistochemical staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.

Anti-KLF5 Antibody

HPA040398
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKLF5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KLF5
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID688
  • Target name
    KLF5
  • Target description
    KLF transcription factor 5
  • Target synonyms
    BTEB2, CKLF, IKLF, Krueppel-like factor 5, (intestinal Kruppel-like factor, BTE-binding protein 2, GC box binding protein 2, Kruppel like factor 5, Kruppel-like factor 5 (intestinal), basic transcription element binding protein 2, colon krueppel-like factor, colon kruppel-like factor, epididymis secretory sperm binding protein, intestinal-enriched krueppel-like factor, intestinal-enriched kruppel-like factor, transcription factor BTEB2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13887
  • Protein Name
    Krueppel-like factor 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of KLF5 using anti-KLF5 antibody (PA1889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF5 antigen affinity purified polyclonal antibody (Catalog # PA1889) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF5 at approximately 60 kDa. The expected band size for KLF5 is at 51 kDa.
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