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KLF5 antibody

GTX103289
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetKLF5
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Overview

  • Supplier
    GeneTex
  • Product Name
    KLF5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID688
  • Target name
    KLF5
  • Target description
    Kruppel like factor 5
  • Target synonyms
    (intestinal Kruppel-like factor; basic transcription element binding protein 2; BTEB2; BTE-binding protein 2; CKLF; colon krueppel-like factor; colon kruppel-like factor; epididymis secretory sperm binding protein; GC box binding protein 2; IKLF; intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); transcription factor BTEB2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13887
  • Protein Name
    Krueppel-like factor 5
  • Scientific Description
    This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • BGP-15 Inhibits Hyperglycemia-Aggravated VSMC Calcification Induced by High Phosphate. Nagy A et al., 2021 Aug 26, Int J Mol Sci
    Read more
  • KLF5-mediated Eppk1 expression promotes cell proliferation in cervical cancer via the p38 signaling pathway. Ma D et al., 2021 Apr 8, BMC Cancer
    Read more
  • Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5. Ma D et al., 2020, Front Cardiovasc Med
    Read more
  • Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission. Ma D et al., 2020 Aug, PLoS Biol
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  • Increased AT2R expression is induced by AT1R autoantibody via two axes, Klf-5/IRF-1 and circErbB4/miR-29a-5p, to promote VSMC migration. Sun Y et al., 2020 Jun 8, Cell Death Dis
    Read more
  • Krueppel Like Factors Family Expression in Cervical Cancer Cells. Marrero-Rodriguez D et al., 2017 May, Arch Med Res
    Read more
  • KLF5 promotes cervical cancer proliferation, migration and invasion in a manner partly dependent on TNFRSF11a expression. Ma D et al., 2017 Nov 16, Sci Rep
    Read more
  • Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming. Miura S et al., 2017 Oct 5, Cell Stem Cell
    Read more
  • iNOS-derived peroxynitrite mediates high glucose-induced inflammatory gene expression in vascular smooth muscle cells through promoting KLF5 expression and nitration. Zhang ML et al., 2017 Nov, Biochim Biophys Acta Mol Basis Dis
    Read more
  • Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages. Jiang W et al., 2016, Am J Transl Res
    Read more

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Figure 1. Western blot analysis of KLF5 using anti-KLF5 antibody (PA1889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF5 antigen affinity purified polyclonal antibody (Catalog # PA1889) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF5 at approximately 60 kDa. The expected band size for KLF5 is at 51 kDa.
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