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KLF1/KLF5/KLF7 Antibody

PACO06456
Assay Genie
ApplicationsImmunoFluorescence, Western Blot, ELISA
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetKLF5
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Overview

  • Supplier
    Assay Genie
  • Product Name
    KLF1/KLF5/KLF7 Antibody (PACO06456)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    WB, IF, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID688
  • Target name
    KLF5
  • Target description
    Kruppel like factor 5
  • Target synonyms
    (intestinal Kruppel-like factor; basic transcription element binding protein 2; BTEB2; BTE-binding protein 2; CKLF; colon krueppel-like factor; colon kruppel-like factor; epididymis secretory sperm binding protein; GC box binding protein 2; IKLF; intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); transcription factor BTEB2
  • Host
    Rabbit
  • Protein IDO75840
  • Protein Name
    Krueppel-like factor 7
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KLF5 using anti-KLF5 antibody (PA1889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF5 antigen affinity purified polyclonal antibody (Catalog # PA1889) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF5 at approximately 60 kDa. The expected band size for KLF5 is at 51 kDa.
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