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Immunohistochemical staining of human oral mucosa shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows moderate cytoplasmic positivity in squamous epithelial cells.

Anti-KRT4 Antibody

HPA034881
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetKRT4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KRT4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3851
  • Target name
    KRT4
  • Target description
    keratin 4
  • Target synonyms
    CK4; CK-4; CYK4; cytokeratin 4; K4; keratin 4, type II; keratin, type II cytoskeletal 4; type-II keratin Kb4; WSN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19013
  • Protein Name
    Keratin, type II cytoskeletal 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 4/KRT4 antigen affinity purified polyclonal antibody (Catalog # A07410-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 4/KRT4 at approximately 56 kDa. The expected band size for Cytokeratin 4/KRT4 is at 56 kDa.
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