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ProductsBack/KRT4 / CK4 / Cytokeratin 4 Antibody (clone ABT-CK4)

KRT4 / CK4 / Cytokeratin 4 Antibody (clone ABT-CK4)

LS-C744498
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetKRT4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KRT4 / CK4 / Cytokeratin 4 Antibody (clone ABT-CK4)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Esophageal squamous carcinoma. Resulting localization is Cytoplasmic, Membranous
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:200 - 1:400) Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Esophageal squamous carcinoma. Resulting localization is Cytoplasmic, Membranous
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABT-CK4
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3851
  • Target name
    KRT4
  • Target description
    keratin 4
  • Target synonyms
    CK-4, CK4, CYK4, K4, WSN1, keratin, type II cytoskeletal 4, cytokeratin 4, keratin 4, type II, type-II keratin Kb4
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 4/KRT4 antigen affinity purified polyclonal antibody (Catalog # A07410-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 4/KRT4 at approximately 56 kDa. The expected band size for Cytokeratin 4/KRT4 is at 56 kDa.
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