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Cytokeratin 4 Antibody (YA1762, Rabbit)

HY-P82017
MedChem Express
TargetKRT4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Cytokeratin 4 Antibody (YA1762, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3851
  • Target name
    KRT4
  • Target description
    keratin 4
  • Target synonyms
    CK4; CK-4; CYK4; cytokeratin 4; K4; keratin 4, type II; keratin, type II cytoskeletal 4; type-II keratin Kb4; WSN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Cytokeratin 4 Antibody (YA1762) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1762), targeting Cytokeratin 4, with a predicted molecular weight of 56 kDa (observed band size: 56 kDa). Cytokeratin 4 Antibody (YA1762) can be used for WB, IP experiment in human, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 4/KRT4 antigen affinity purified polyclonal antibody (Catalog # A07410-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 4/KRT4 at approximately 56 kDa. The expected band size for Cytokeratin 4/KRT4 is at 56 kDa.
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