Bio-Connect

Anti-KRT8 Antibody

130-10237
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-KRT8 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Scientific Description
    Rabbit anti-KRT8 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetKRT8
  • SizePrice
Product group Antibodies
Assay Genie
TargetKRT8
  • SizePrice
Formalin-fixed and rat colon tissue labeled with Anti-CK8 Polyclonal Antibody, Unconjugated (bs-1106R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT8
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
TargetKRT8
  • SizePrice
Figure 1. Western blot analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (A01421). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: A549 whole cell lysates, Lane 6: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 8 antigen affinity purified polyclonal antibody (Catalog # A01421) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD, 56KD. The expected band size for Cytokeratin 8 is at 54KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetKRT8
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetKRT8
  • SizePrice
Anti-Keratin 8 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetKRT8
  • SizePrice
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetKRT8
  • SizePrice