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Anti-Lactate Dehydrogenase B (LDHB) Monoclonal Antibody

CAU36538
Biomatik
ApplicationsFlow Cytometry
Product group Antibodies
TargetLDHB
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Overview

  • Supplier
    Biomatik
  • Product Name
    Anti-Lactate Dehydrogenase B (LDHB) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C16
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3945
  • Target name
    LDHB
  • Target description
    lactate dehydrogenase B
  • Target synonyms
    HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46, testicular secretory protein Li 25
  • Host
    Rabbit
  • Isotype
    IgG1
  • Protein IDP07195
  • Protein Name
    L-lactate dehydrogenase B chain
  • Scientific Description
    The Anti-Lactate Dehydrogenase B (LDHB) Monoclonal Antibody (Species: Human) has been validated for the following applications: FCM.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of LDHB using anti-LDHB antibody (PB10076). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse kidney tissue lysates, Lane 11: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHB antigen affinity purified polyclonal antibody (Catalog # PB10076) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDHB at approximately 37 kDa. The expected band size for LDHB is at 37 kDa.
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