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ProductsBack/LDHB Polyclonal Antibody
Lane 1: 293T cell lysates; Lane 2: LDHB knockout (KO) 293T cell lysates probed with LDHB Polyclonal Antibody, Unconjugated (bs-55121R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: LDHB knockout (KO) 293T cell lysates probed with LDHB Polyclonal Antibody, Unconjugated (bs-55121R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: LDHB knockout (KO) 293T cell lysates probed with LDHB Polyclonal Antibody, Unconjugated (bs-55121R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

LDHB Polyclonal Antibody

BS-55121R
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLDHB
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Overview

  • Supplier
    Bioss
  • Product Name
    LDHB Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3945
  • Target name
    LDHB
  • Target description
    lactate dehydrogenase B
  • Target synonyms
    HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46, testicular secretory protein Li 25
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07195
  • Protein Name
    L-lactate dehydrogenase B chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of LDHB using anti-LDHB antibody (PB10076). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse kidney tissue lysates, Lane 11: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHB antigen affinity purified polyclonal antibody (Catalog # PB10076) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDHB at approximately 37 kDa. The expected band size for LDHB is at 37 kDa.
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