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ProductsBack/Goat anti-LDHB (aa15-25)

Goat anti-LDHB (aa15-25)

EB12394
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLDHB
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-LDHB (aa15-25) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3945
  • Target name
    LDHB
  • Target description
    lactate dehydrogenase B
  • Target synonyms
    HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46, testicular secretory protein Li 25
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002291.1. Purification: Antigen affinity purified. Names and symbols: LDHB; lactate dehydrogenase B; LDH-B; LDH-H; LDHBD; TRG-5; L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LDHB using anti-LDHB antibody (PB10076). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse kidney tissue lysates, Lane 11: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHB antigen affinity purified polyclonal antibody (Catalog # PB10076) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDHB at approximately 37 kDa. The expected band size for LDHB is at 37 kDa.
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