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Rat anti Laminin

MUB1100P
Nordic-MUbio
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetLAMC1
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rat anti Laminin
  • Delivery Days Customer
    7
  • Application Supplier Note
    A5 is suitable for immunocytochemistry, immunofluorescence and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Immunocytochemistry;Immunohistochemistry (frozen);Immunofluorescence
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A5
  • Gene ID3915
  • Target name
    LAMC1
  • Target description
    laminin subunit gamma 1
  • Target synonyms
    LAMB2, laminin subunit gamma-1, S-LAM gamma, S-laminin subunit gamma, laminin B2 chain, laminin, gamma 1 (formerly LAMB2), laminin-10 subunit gamma, laminin-11 subunit gamma, laminin-2 subunit gamma, laminin-3 subunit gamma, laminin-4 subunit gamma, laminin-6 subunit gamma, laminin-7 subunit gamma, laminin-8 subunit gamma, laminin-9 subunit gamma
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP11047
  • Protein Name
    Laminin subunit gamma-1
  • Source
    A5 is a rat monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with spleen cells from a Fisher rat immunized with a laminin preparation from the EHS mouse tumor.
  • Reactivity Supplier
    Human;Mouse;Swine
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Laminin Gamma 1/LAMC1 using anti-Laminin Gamma 1/LAMC1 antibody (A03522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin Gamma 1/LAMC1 antigen affinity purified polyclonal antibody (Catalog # A03522-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin Gamma 1/LAMC1 at approximately 200 kDa. The expected band size for Laminin Gamma 1/LAMC1 is at 178 kDa.
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