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Anti-LgR5 [1-12]

AB04170-11.0
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLGR5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-LgR5 [1-12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis. The original format of the antibody could recognise human LgR5 expressed in 293 cells by FACS. The original format of the antibody was used to detect expression of LgR5 on normal human tissues by immunohistochemistry. The antibody stained normal intestinal crypts in the expected pattern. The antibody also showed moderate staining in hair follicle, and weak staining in fallopian tube, endometrium, adrenal gland, and spinal cord. Further, the antibody detected LgR5 in different colon cancer cell lines (SW403, SW948, CACO-2, T84, KM-12, C2BBel, SW1463, SK-CO-1, LOVO) by immunohistochemistry (US20190248889).
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1-12
  • Gene ID8549
  • Target name
    LGR5
  • Target description
    leucine rich repeat containing G protein-coupled receptor 5
  • Target synonyms
    FEX; GPR49; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; GRP49; HG38; leucine-rich repeat-containing G-protein coupled receptor 5; orphan G protein-coupled receptor HG38
  • Host
    Human
  • Isotype
    IgG2
  • Protein IDO75473
  • Protein Name
    Leucine-rich repeat-containing G-protein coupled receptor 5
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA012906LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of LGR5 using anti-LGR5 antibody (A00239-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGR5 antigen affinity purified polyclonal antibody (Catalog # A00239-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LGR5 at approximately 130 kDa. The expected band size for LGR5 is at 100,97,92 kDa.
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