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Anti-LIFR Antibody

Anti-LIFR Antibody

A101396
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-LIFR Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to LIFR.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIFR using anti-LIFR antibody (PB9661). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: SW620 Whole Cell Lysate at 40ug, Lane 2: COLO320 Whole Cell Lysate at 40ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIFR antigen affinity purified polyclonal antibody (Catalog # PB9661) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIFR at approximately 190 kDa. The expected band size for LIFR is at 190 kDa.
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