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Western Blot analysis of 293 cells using LIFR Polyclonal Antibody
Western Blot analysis of 293 cells using LIFR Polyclonal Antibody
Western Blot analysis of 293 cells using LIFR Polyclonal Antibody

LIFR Antibody

CSB-PA006260
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetLIFR
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Overview

  • Supplier
    Cusabio
  • Product Name
    LIFR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3977
  • Target name
    LIFR
  • Target description
    LIF receptor subunit alpha
  • Target synonyms
    CD118; CD118 antigen; leukemia inhibitory factor receptor; leukemia inhibitory factor receptor alpha; LIF receptor alpha; LIF-R; SJS2; STWS; SWS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42702
  • Protein Name
    Leukemia inhibitory factor receptor
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LIFR using anti-LIFR antibody (PB9661). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: SW620 Whole Cell Lysate at 40ug, Lane 2: COLO320 Whole Cell Lysate at 40ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIFR antigen affinity purified polyclonal antibody (Catalog # PB9661) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIFR at approximately 190 kDa. The expected band size for LIFR is at 190 kDa.
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Human U937 cells probed with LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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