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Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Anti-LIG4 Antibody

HPA001334
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLIG4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LIG4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3981
  • Target name
    LIG4
  • Target description
    DNA ligase 4
  • Target synonyms
    LIG4S, DNA ligase 4, DNA joinase, DNA ligase IV, DNA repair enzyme, ligase IV, DNA, ATP-dependent, polydeoxyribonucleotide synthase [ATP] 4, polynucleotide ligase, sealase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49917
  • Protein Name
    DNA ligase 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNA Ligase IV/LIG4 using anti-DNA Ligase IV/LIG4 antibody (A01685-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Ligase IV/LIG4 antigen affinity purified polyclonal antibody (Catalog # A01685-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Ligase IV/LIG4 at approximately 104 kDa. The expected band size for DNA Ligase IV/LIG4 is at 104 kDa.
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