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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.

Anti-LIG4 Antibody

HPA057325
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLIG4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LIG4
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3981
  • Target name
    LIG4
  • Target description
    DNA ligase 4
  • Target synonyms
    LIG4S, DNA ligase 4, DNA joinase, DNA ligase IV, DNA repair enzyme, ligase IV, DNA, ATP-dependent, polydeoxyribonucleotide synthase [ATP] 4, polynucleotide ligase, sealase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49917
  • Protein Name
    DNA ligase 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of DNA Ligase IV/LIG4 using anti-DNA Ligase IV/LIG4 antibody (A01685-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Ligase IV/LIG4 antigen affinity purified polyclonal antibody (Catalog # A01685-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Ligase IV/LIG4 at approximately 104 kDa. The expected band size for DNA Ligase IV/LIG4 is at 104 kDa.
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Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Jurkat Cells; Probed with DNA Ligase IV (2D9) Monoclonal Antibody (bsm-54131R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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