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Immunohistochemical staining of human duodenum shows both membranous and nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows both membranous and nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows both membranous and nuclear positivity in glandular cells.

Anti-LMO7 Antibody

HPA020923
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLMO7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LMO7
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4008
  • Target name
    LMO7
  • Target description
    LIM domain 7
  • Target synonyms
    F-box only protein 20; F-box protein Fbx20; FBX20; FBXO20; LIM domain only 7 protein; LIM domain only protein 7; LMO-7; LMO7b; LOMP; zinc-finger domain-containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WWI1
  • Protein Name
    LIM domain only protein 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LMO7 using anti-LMO7 antibody (A05712-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO7 antigen affinity purified polyclonal antibody (Catalog # A05712-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO7 at approximately 150 kDa. The expected band size for LMO7 is at 193 kDa.
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