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Immunofluorescent staining of human cell line A-431 shows localization to actin filaments.
Immunofluorescent staining of human cell line A-431 shows localization to actin filaments.
Immunofluorescent staining of human cell line A-431 shows localization to actin filaments.

Anti-LMO7 Antibody

HPA050184
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLMO7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LMO7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4008
  • Target name
    LMO7
  • Target description
    LIM domain 7
  • Target synonyms
    FBX20, FBXO20, LMO7b, LOMP, LIM domain only protein 7, F-box only protein 20, F-box protein Fbx20, LIM domain only 7 protein, LMO-7, zinc-finger domain-containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WWI1
  • Protein Name
    LIM domain only protein 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LMO7 using anti-LMO7 antibody (A05712-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO7 antigen affinity purified polyclonal antibody (Catalog # A05712-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO7 at approximately 150 kDa. The expected band size for LMO7 is at 193 kDa.
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IHC image of CSB-PA823897LA01HU diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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