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Anti-LOC134147/CMBL Antibody

ORB2604276
Biorbyt
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCMBL
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-LOC134147/CMBL Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.1-0.25microg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5microg/ml, Human Flow Cytometry (Fixed), 1-3microg/1x106 cells, Human ELISA, 0.1-0.5microg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, FC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    FITC
  • Gene ID134147
  • Target name
    CMBL
  • Target description
    carboxymethylenebutenolidase homolog
  • Target synonyms
    JS-1, carboxymethylenebutenolidase homolog, carboxymethylenebutenolidase homolog (Pseudomonas), carboxymethylenebutenolidase-like (Pseudomonas)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DG6
  • Protein Name
    Carboxymethylenebutenolidase homolog
  • Scientific Description
    Anti-LOC134147/CMBL Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (A04921-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human U87 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # A04921-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD.
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