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Anti-LPP Antibody

Anti-LPP Antibody

A39171
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-LPP Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.9 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to LPP
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA065419(LPP Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LPP Monoclonal Antibody, Unconjugated (bsm-54756R) at 1:200 for 30 minutes at room temperature, DAB staining.
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Figure 1. Western blot analysis of LPP using anti-LPP antibody (A01240-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPP antigen affinity purified polyclonal antibody (Catalog # A01240-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPP at approximately 75KD. The expected band size for LPP is at 75KD.
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