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ICC/IF analysis of HeLa cells using GTX83092 LPP antibody [8B3A11]. Green : LPP Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of HeLa cells using GTX83092 LPP antibody [8B3A11]. Green : LPP Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of HeLa cells using GTX83092 LPP antibody [8B3A11]. Green : LPP Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments

LPP antibody [8B3A11]

GTX83092
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLPP
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Overview

  • Supplier
    GeneTex
  • Product Name
    LPP antibody [8B3A11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8B3A11
  • Conjugate
    Unconjugated
  • Gene ID4026
  • Target name
    LPP
  • Target description
    LIM domain containing preferred translocation partner in lipoma
  • Target synonyms
    LIM protein; lipoma preferred partner; lipoma-preferred partner
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ93052
  • Protein Name
    Lipoma-preferred partner
  • Scientific Description
    This gene encodes a member of a subfamily of LIM domain proteins that are characterized by an N-terminal proline-rich region and three C-terminal LIM domains. The encoded protein localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility. This protein also shuttles through the nucleus and may function as a transcriptional co-activator. This gene is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LPP using anti-LPP antibody (A01240-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPP antigen affinity purified polyclonal antibody (Catalog # A01240-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPP at approximately 75KD. The expected band size for LPP is at 75KD.
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