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ProductsBack/Goat anti-LPP

Goat anti-LPP

EB09755
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLPP
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-LPP Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4026
  • Target name
    LPP
  • Target description
    LIM domain containing preferred translocation partner in lipoma
  • Target synonyms
    LIM protein; lipoma preferred partner; lipoma-preferred partner
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005569.1. Purification: Antigen affinity purified. Names and symbols: LPP; LIM domain containing preferred translocation partner in lipoma; LIM domain-containing preferred translocation partner in lipoma; LIM protein; Lipoma-preferred partner
  • Reactivity Supplier
    Human, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of LPP using anti-LPP antibody (A01240-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPP antigen affinity purified polyclonal antibody (Catalog # A01240-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPP at approximately 75KD. The expected band size for LPP is at 75KD.
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