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Immunohistochemical staining of human cerebellum shows very strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows very strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows very strong cytoplasmic positivity in Purkinje cells.

Anti-LRIG1 Antibody

HPA011846
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLRIG1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LRIG1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26018
  • Target name
    LRIG1
  • Target description
    leucine rich repeats and immunoglobulin like domains 1
  • Target synonyms
    LIG-1, LIG1, leucine-rich repeats and immunoglobulin-like domains protein 1, leucine-rich repeat protein LRIG1, ortholog of mouse integral membrane glycoprotein LIG-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96JA1
  • Protein Name
    Leucine-rich repeats and immunoglobulin-like domains protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LRIG1 using anti-LRIG1 antibody (A04613-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIG1 antigen affinity purified polyclonal antibody (Catalog # A04613-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIG1 at approximately 145 kDa. The expected band size for LRIG1 is at 119 kDa.
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