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ProductsBack/LRIG1 antibody [C2C3], C-term

LRIG1 antibody [C2C3], C-term

GTX119485
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLRIG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    LRIG1 antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.61 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID26018
  • Target name
    LRIG1
  • Target description
    leucine rich repeats and immunoglobulin like domains 1
  • Target synonyms
    LIG-1, LIG1, leucine-rich repeats and immunoglobulin-like domains protein 1, leucine-rich repeat protein LRIG1, ortholog of mouse integral membrane glycoprotein LIG-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96JA1
  • Protein Name
    Leucine-rich repeats and immunoglobulin-like domains protein 1
  • Scientific Description
    Act as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hsu HL, Chen HK, Tsai CH, et al. Aryl Hydrocarbon Receptor Defect Attenuates Mitogen-Activated Signaling through Leucine-Rich Repeats and Immunoglobulin-like Domains 1 (LRIG1)-Dependent EGFR Degradation. Int J Mol Sci. 2021,22(18). doi: 10.3390/ijms22189988
    Read this paper
  • Yokdang N, Hatakeyama J, Wald JH, et al. LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells. Oncogene. 2016,35(22):2932-47. doi: 10.1038/onc.2015.345
    Read this paper

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Figure 1. Western blot analysis of LRIG1 using anti-LRIG1 antibody (A04613-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIG1 antigen affinity purified polyclonal antibody (Catalog # A04613-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIG1 at approximately 145 kDa. The expected band size for LRIG1 is at 119 kDa.
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