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Anti-LRPPRC Antibody

Anti-LRPPRC Antibody

A45492
Antibodies.com
ApplicationsImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-LRPPRC Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.2 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to LRPPRC
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate All lanes: LRPPRC antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 158 kDa Observed band size: 158 kDa
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Figure 1. Western blot analysis of LRPPRC using anti-LRPPRC antibody (A03264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: monkey heart tissue lysates, Lane 7: rat heart tissue lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRPPRC antigen affinity purified polyclonal antibody (Catalog # A03264) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRPPRC at approximately 157KD. The expected band size for LRPPRC is at 150-160KD.
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