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LRPPRC Antibody

LS-C332518
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLRPPRC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LRPPRC Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 157kDa, while the observed MW by Western blot was 140kDa.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:100 - 1:200), IP (1:20 - 1:50), WB (1:1000 - 1:2000) The predicted MW is 157kDa, while the observed MW by Western blot was 140kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10128
  • Target name
    LRPPRC
  • Target description
    leucine rich pentatricopeptide repeat containing
  • Target synonyms
    CLONE-23970, GP130, LRP130, LSFC, MC4DN5, leucine-rich PPR motif-containing protein, mitochondrial, 130 kDa leucine-rich protein, LRP 130, leucine-rich PPR-motif containing, mitochondrial leucine-rich PPR motif-containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of LRPPRC using anti-LRPPRC antibody (A03264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: monkey heart tissue lysates, Lane 7: rat heart tissue lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRPPRC antigen affinity purified polyclonal antibody (Catalog # A03264) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRPPRC at approximately 157KD. The expected band size for LRPPRC is at 150-160KD.
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