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Anti-LUM Antibody

144-05352
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetLUM
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-LUM Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4060
  • Target name
    LUM
  • Target description
    lumican
  • Target synonyms
    epididymis secretory sperm binding protein; keratan sulfate proteoglycan lumican; KSPG lumican; LDC; lumican; lumican proteoglycan; SLRR2D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51884
  • Protein Name
    Lumican
  • Scientific Description
    LUM Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lumican using anti-Lumican antibody (A01034-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysate, Lane 3: human CCRF-CEM whole cell lysate, Lane 4: human Hela whole cell lysate, Lane 5: human Jurkat whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican antigen affinity purified polyclonal antibody (Catalog # A01034-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lumican at approximately 58KD. The expected band size for Lumican is at 38KD.
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