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Immunohistochemical staining of human breast shows moderate to strong cytoplasmic positivity.
Immunohistochemical staining of human breast shows moderate to strong cytoplasmic positivity.
Immunohistochemical staining of human breast shows moderate to strong cytoplasmic positivity.

Anti-LUM Antibody

HPA001522
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLUM
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LUM Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4060
  • Target name
    LUM
  • Target description
    lumican
  • Target synonyms
    LDC, SLRR2D, lumican, KSPG lumican, epididymis secretory sperm binding protein, keratan sulfate proteoglycan lumican, lumican proteoglycan
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51884
  • Protein Name
    Lumican
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Lumican using anti-Lumican antibody (A01034-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysate, Lane 3: human CCRF-CEM whole cell lysate, Lane 4: human Hela whole cell lysate, Lane 5: human Jurkat whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican antigen affinity purified polyclonal antibody (Catalog # A01034-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lumican at approximately 58KD. The expected band size for Lumican is at 38KD.
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