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Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.

Anti-MADCAM1 Antibody

HPA077998
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMADCAM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MADCAM1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8174
  • Target name
    MADCAM1
  • Target description
    mucosal vascular addressin cell adhesion molecule 1
  • Target synonyms
    hMAdCAM-1; MACAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13477
  • Protein Name
    Mucosal addressin cell adhesion molecule 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAdCAM1 using anti-MAdCAM1 antibody (A04227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate, Lane 2: human A375 whole cell lysate, Lane 3: human K562 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAdCAM1 antigen affinity purified polyclonal antibody (Catalog # A04227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAdCAM1 at approximately 43KD. The expected band size for MAdCAM1 is at 40KD.
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