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MAdCAM-1 Antibody

LS-C408632
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMADCAM1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAdCAM-1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 21kDa/28kDa/31kDa/40kDa, while the observed MW by Western blot was Refer to Figures.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:200), WB (1:200 - 1:1000) The predicted MW is 21kDa/28kDa/31kDa/40kDa, while the observed MW by Western blot was Refer to Figures.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8174
  • Target name
    MADCAM1
  • Target description
    mucosal vascular addressin cell adhesion molecule 1
  • Target synonyms
    hMAdCAM-1; MACAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAdCAM1 using anti-MAdCAM1 antibody (A04227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate, Lane 2: human A375 whole cell lysate, Lane 3: human K562 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAdCAM1 antigen affinity purified polyclonal antibody (Catalog # A04227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAdCAM1 at approximately 43KD. The expected band size for MAdCAM1 is at 40KD.
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