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Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-MAdCAM1 Polyclonal Antibody, Unconjugated (bs-11179R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-MAdCAM1 Polyclonal Antibody, Unconjugated (bs-11179R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-MAdCAM1 Polyclonal Antibody, Unconjugated (bs-11179R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n

MAdCAM1 Polyclonal Antibody

BS-11179R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMADCAM1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MAdCAM1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8174
  • Target name
    MADCAM1
  • Target description
    mucosal vascular addressin cell adhesion molecule 1
  • Target synonyms
    hMAdCAM-1; MACAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13477
  • Protein Name
    Mucosal addressin cell adhesion molecule 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAdCAM1 using anti-MAdCAM1 antibody (A04227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate, Lane 2: human A375 whole cell lysate, Lane 3: human K562 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAdCAM1 antigen affinity purified polyclonal antibody (Catalog # A04227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAdCAM1 at approximately 43KD. The expected band size for MAdCAM1 is at 40KD.
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