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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-MAT1A Antibody

HPA048627
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAT1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAT1A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4143
  • Target name
    MAT1A
  • Target description
    methionine adenosyltransferase 1A
  • Target synonyms
    MAT, MATA1, SAMS, SAMS1, S-adenosylmethionine synthase isoform type-1, MAT 1, MAT-I/III, adoMet synthase 1, adoMet synthetase 1, methionine adenosyltransferase 1, methionine adenosyltransferase I, alpha, methionine adenosyltransferase I/III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00266
  • Protein Name
    S-adenosylmethionine synthase isoform type-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MAT1A using anti-MAT1A antibody (A04203-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT1A antigen affinity purified polyclonal antibody (Catalog # A04203-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT1A at approximately 55 kDa. The expected band size for MAT1A is at 44 kDa.
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