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MAT1A Antibody (YA3199, Rabbit)

HY-P83454
MedChem Express
TargetMAT1A
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MAT1A Antibody (YA3199, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4143
  • Target name
    MAT1A
  • Target description
    methionine adenosyltransferase 1A
  • Target synonyms
    MAT, MATA1, SAMS, SAMS1, S-adenosylmethionine synthase isoform type-1, MAT 1, MAT-I/III, adoMet synthase 1, adoMet synthetase 1, methionine adenosyltransferase 1, methionine adenosyltransferase I, alpha, methionine adenosyltransferase I/III
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MAT1A Antibody (YA3199) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3199), targeting MAT1A, with a predicted molecular weight of 44 kDa (observed band size: 44 kDa). MAT1A Antibody (YA3199) can be used for WB, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAT1A using anti-MAT1A antibody (A04203-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT1A antigen affinity purified polyclonal antibody (Catalog # A04203-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT1A at approximately 55 kDa. The expected band size for MAT1A is at 44 kDa.
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