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Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.

Anti-MAT2B Antibody

HPA037722
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAT2B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAT2B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27430
  • Target name
    MAT2B
  • Target description
    methionine adenosyltransferase 2 non-catalytic beta subunit
  • Target synonyms
    MAT-II, MATIIbeta, Nbla02999, SDR23E1, TGR, methionine adenosyltransferase 2 subunit beta, MAT II beta, beta regulatory subunit of methionine adenosyltransferase, dTDP-4-keto-6-deoxy-D-glucose 4-reductase, methionine adenosyltransferase 2B, methionine adenosyltransferase II, beta, putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, putative protein product of Nbla02999, short chain dehydrogenase/reductase family 23E, member 1, testicular tissue protein Li 118
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZL9
  • Protein Name
    Methionine adenosyltransferase 2 subunit beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MAT2B/TGR using anti-MAT2B/TGR antibody (A06719-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2B/TGR antigen affinity purified polyclonal antibody (Catalog # A06719-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT2B/TGR at approximately 38 kDa. The expected band size for MAT2B/TGR is at 38 kDa.
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