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ProductsBack/MAT2B antibody [N3C3]
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MAT2B(GTX115863) antibody at 1:200 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MAT2B(GTX115863) antibody at 1:200 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MAT2B(GTX115863) antibody at 1:200 dilution.

MAT2B antibody [N3C3]

GTX115863
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMAT2B
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAT2B antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.45 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27430
  • Target name
    MAT2B
  • Target description
    methionine adenosyltransferase 2 non-catalytic beta subunit
  • Target synonyms
    MAT-II, MATIIbeta, Nbla02999, SDR23E1, TGR, methionine adenosyltransferase 2 subunit beta, MAT II beta, beta regulatory subunit of methionine adenosyltransferase, dTDP-4-keto-6-deoxy-D-glucose 4-reductase, methionine adenosyltransferase 2B, methionine adenosyltransferase II, beta, putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, putative protein product of Nbla02999, short chain dehydrogenase/reductase family 23E, member 1, testicular tissue protein Li 118
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZL9
  • Protein Name
    Methionine adenosyltransferase 2 subunit beta
  • Scientific Description
    The protein encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Fisslthaler B, Zippel N, Abdel Malik R, et al. Myeloid-Specific Deletion of the AMPKα2 Subunit Alters Monocyte Protein Expression and Atherogenesis. Int J Mol Sci. 2019,20(12). doi: 10.3390/ijms20123005
    Read this paper

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Figure 1. Western blot analysis of MAT2B/TGR using anti-MAT2B/TGR antibody (A06719-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2B/TGR antigen affinity purified polyclonal antibody (Catalog # A06719-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT2B/TGR at approximately 38 kDa. The expected band size for MAT2B/TGR is at 38 kDa.
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