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Immunofluorescent analysis of HepG2 cells using CSB-PA882157LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using CSB-PA882157LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using CSB-PA882157LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

MAT2B Antibody

CSB-PA882157LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetMAT2B
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAT2B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27430
  • Target name
    MAT2B
  • Target description
    methionine adenosyltransferase 2 non-catalytic beta subunit
  • Target synonyms
    MAT-II, MATIIbeta, Nbla02999, SDR23E1, TGR, methionine adenosyltransferase 2 subunit beta, MAT II beta, beta regulatory subunit of methionine adenosyltransferase, dTDP-4-keto-6-deoxy-D-glucose 4-reductase, methionine adenosyltransferase 2B, methionine adenosyltransferase II, beta, putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, putative protein product of Nbla02999, short chain dehydrogenase/reductase family 23E, member 1, testicular tissue protein Li 118
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZL9
  • Protein Name
    Methionine adenosyltransferase 2 subunit beta
  • Scientific Description
    Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S-adenosylmethionine inhibition.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAT2B/TGR using anti-MAT2B/TGR antibody (A06719-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2B/TGR antigen affinity purified polyclonal antibody (Catalog # A06719-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT2B/TGR at approximately 38 kDa. The expected band size for MAT2B/TGR is at 38 kDa.
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