Figure 1. Western blot analysis of MED13 using anti-MED13 antibody (A04545-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED13 antigen affinity purified polyclonal antibody (Catalog # A04545-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED13 at approximately 300KD. The expected band size for MED13 is at 239KD.
Figure 1. Western blot analysis of MED13 using anti-MED13 antibody (A04545-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED13 antigen affinity purified polyclonal antibody (Catalog # A04545-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED13 at approximately 300KD. The expected band size for MED13 is at 239KD.
Anti-MED13 Antibody Picoband(r)
A04545-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMED13
Overview
- SupplierBoster Bio
- Product NameAnti-MED13 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID9969
- Target nameMED13
- Target descriptionmediator complex subunit 13
- Target synonymsactivator-recruited cofactor 250 kDa component; ARC250; DRIP250; HSPC221; mediator of RNA polymerase II transcription subunit 13; mediator of RNA polymerase II transcription, subunit 13 homolog; MRD61; THRAP1; thyroid hormone receptor-associated protein 1; thyroid hormone receptor-associated protein complex 240 kDa component; thyroid hormone receptor-associated protein complex component TRAP240; thyroid hormone receptor-associated protein, 240 kDa subunit; TRAP240; vitamin D3 receptor-interacting protein complex component DRIP250
- HostRabbit
- IsotypeIgG
- Protein IDQ9UHV7
- Protein NameMediator of RNA polymerase II transcription subunit 13
- Scientific DescriptionBoster Bio Anti-MED13 Antibody Picoband® catalog # A04545-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203