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ProductsBack/Anti-MEK1/MAP2K1 Antibody Picoband(r)
Anti-MEK1 antibody, PA1376, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: CEM Cell Lysate Lane 4: COLO20 Cell Lysate
Anti-MEK1 antibody, PA1376, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: CEM Cell Lysate Lane 4: COLO20 Cell Lysate
Anti-MEK1 antibody, PA1376, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: CEM Cell Lysate Lane 4: COLO20 Cell Lysate

Anti-MEK1/MAP2K1 Antibody Picoband(r)

PA1376
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMAP2K1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-MEK1 Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHP, ICC, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    Boster Bio Anti-MEK1/MAP2K1 Antibody catalog # PA1376. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice.
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Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
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