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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Anti-MGAT3 Antibody

HPA017598
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMGAT3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MGAT3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4248
  • Target name
    MGAT3
  • Target description
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • Target synonyms
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; GlcNAc-T III; GNT3; GNT-III; mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; N-acetylglucosaminyltransferase III; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ09327
  • Protein Name
    Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MGAT3 using anti-MGAT3 antibody (A08470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGAT3 antigen affinity purified polyclonal antibody (Catalog # A08470-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGAT3 at approximately 61 kDa. The expected band size for MGAT3 is at 61 kDa.
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