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GNT-III / MGAT3 Antibody

LS-C409670
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMGAT3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GNT-III / MGAT3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 61kDa, while the observed MW by Western blot was 61kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 61kDa, while the observed MW by Western blot was 61kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4248
  • Target name
    MGAT3
  • Target description
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • Target synonyms
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; GlcNAc-T III; GNT3; GNT-III; mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; N-acetylglucosaminyltransferase III; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MGAT3 using anti-MGAT3 antibody (A08470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGAT3 antigen affinity purified polyclonal antibody (Catalog # A08470-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGAT3 at approximately 61 kDa. The expected band size for MGAT3 is at 61 kDa.
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