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Western Blot Positive WB detected in: Mouse kidney tissue All lanes: MGAT3 antibody at 2.35ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
Western Blot Positive WB detected in: Mouse kidney tissue All lanes: MGAT3 antibody at 2.35ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
Western Blot Positive WB detected in: Mouse kidney tissue All lanes: MGAT3 antibody at 2.35ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa

MGAT3 Antibody

CSB-PA22969A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMGAT3
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Overview

  • Supplier
    Cusabio
  • Product Name
    MGAT3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4248
  • Target name
    MGAT3
  • Target description
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • Target synonyms
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; GlcNAc-T III; GNT3; GNT-III; mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; N-acetylglucosaminyltransferase III; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ09327
  • Protein Name
    Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • Scientific Description
    It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MGAT3 using anti-MGAT3 antibody (A08470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGAT3 antigen affinity purified polyclonal antibody (Catalog # A08470-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGAT3 at approximately 61 kDa. The expected band size for MGAT3 is at 61 kDa.
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